Posted on October 4, 2019

ACTINOMADURA MADURAE PDF

Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. It has not previously been reported. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and. Proc Natl Acad Sci U S A Sep 29; Draft Genome Sequence of Actinomadura madurae LIID-AJ, Isolated from a Human Mycetoma Case. Vera-Cabrera L.

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Tracking a hospital outbreak of carbapenem-resistant Klebsiella pneumoniae with whole-genome sequencing. Formalin-fixed paraffin-embedded FFPE material from the biopsy specimen was concurrently submitted for molecular characterization of the pathogen to the University of Washington Molecular Diagnosis Microbiology Section.

Nonmycetomic Actinomadura madurae infection in a patient with AIDS.

The consensus sequence determined in this work has been submitted to GenBank under accession no. For some sequences, multiple organisms met the identity threshold for species-level classification; in such cases, species names separated by a slash and genus names separated by a semicolon list the possible taxonomic assignments. Bacterial community shift in treated periodontitis patients revealed by ion torrent 16S rRNA gene amplicon sequencing.

Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections. However, on imaging, the patient’s osteomyelitis was found to involve all bones of the foot, including the calcaneus.

QIDciprofloxacin mg p. Images in this article Image on p. Published ahead of print 9 October Associated Data Supplementary Materials. Cummingsa Bronwyn H. Acitnomadura year-old woman from northern Mexico with a diagnosis of diabetes presented with a year history of a gradually enlarging right foot demonstrating multiple draining lesions.

Nonmycetomic Actinomadura madurae infection in a patient with AIDS.

Cooksonmaduraae, b madkrae Noah G. Given the extent of infection, neither additional antibiotic therapy nor debridement with subsequent reconstruction was felt to be a viable option, and a recommendation was made for below-the-knee amputation.

Deep sequencing successfully detected the presence of A. Molecular-genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis. Polymicrobial specimens may be difficult or even impossible to fully characterize by techniques in common clinical use: The isolate from the patient’s blood was subjected to dilutional antimicrobial susceptibility tests with 12 antimicrobial agents and was found to have a wide spectrum of susceptibility.

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Actinomadura madurae (ID ) – Genome – NCBI

The patient noted some retreat of the lesions and improvement in her pain and pruritis while on this treatment, and she was transitioned to oral actnomadura mg p. The tallies and percent abundance of reads corresponding to each organism are indicated. The genus Actinomadura is one of four genera of actinobacteria that belong to the family Thermomonosporaceae.

Author information Article notes Copyright and License information Disclaimer. Madure of aerobic actinomycetes of clinical importance. A systematic approach for discovering novel, clinically relevant bacteria. Fournier PE, Raoult D. Wain J, Mavrogiorgou E. Support Center Support Center.

No new madrae evolved while she was on this therapy, but approximately 1 year prior to presentation the patient self-discontinued ciprofloxacin and shortly thereafter noted increasing drainage from her foot. This article has been cited by other articles in PMC. It contains actinomaduta, Gram-positive, non-acid-fast, non-motile, chemo-organotrophic actinomycetes that produce well-developed, non-fragmenting vegetative mycelia and aerial hyphae that differentiate into surface-ornamented spore chains.

Nevertheless, deep sequencing recovered reads 2. You can help Wikipedia by expanding it. Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. This finding is consistent with the results of both culture and Sanger sequencing for the specimen, which were dominated by S.

It has not previously been reported to have caused invasive pulmonary or disseminated infection in humans. Actinomycetoma in the United States. Views Read Edit View history.

Both of these diagnostic limitations complicated analysis of the patient specimen in this msdurae, a biopsy specimen from a diabetic foot ulcer.

Consequently, the likely causative organism of the patient’s foot infection could actinomaduura be identified by existing clinical diagnostic approaches. Int J Syst Evol Microbiol. The phospholipid pattern is PI diphosphatidylglycerol and phosphatidylinositol are present as major phospholipids and the fatty acid pattern is type 3a branched saturated and unsaturated fatty acids plus tuberculostearic acid.

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The case exemplifies several capabilities of deep sequencing as a clinical diagnostic tool, specifically, deconvoluting the identity of individual organisms within polymicrobial samples, classifying organisms directly from patient specimens without the need for culture, and characterizing nonviable or unculturable organisms in this case, organisms killed by FFPE processing prior to pathology examination.

Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions. Actinobacteria Bacteria genera Actinobacteria stubs.

The Ribosomal Database Project: Although the patient remained ambulatory throughout the course of her disease, she gradually had to modify her footwear to accommodate the increasing size of her foot, which at the time of presentation was causing her great pain. Neoscytalidium dimidiatum and Aspergillus flavus. This unusual microorganism may be a cause of infections in severely immunosuppressed patients.

Comparison of culture and molecular identification of bacteria in chronic wounds. Examination with next-generation sequencing technology of the bacterial microbiota in bronchoalveolar lavage samples after traumatic injury.

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Culture of discharged material reportedly returned with overgrowth of skin flora and provided no evidence of infection with Actinomyces species. Prospects for the future using genomics mwdurae proteomics in clinical microbiology. Although the application of deep sequencing to microbial communities traces its origins to metagenomics research 13we recently demonstrated the feasibility of using deep sequencing to interrogate the composition of polymicrobial specimens in a clinical context by sequencing bacterial 16S rRNA amplified directly from patient material 3.

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