Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method
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Department of Health and Human services publication Biosafety in Microbiological and Biomedical Laboratories should be consulted see Allow agar to gel firmly before inoculating. May be sterilized in 1, mL borosilicate glass flasks and petri dishes poured from this.
In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise. Leonard, Technical Director, at the address shown below. Parallel Streak Method Developed in by AATCC Committee RA31; reaffirmed, ; editorially aatc,; revisedwith title change; editorially revised and reaffirmed Other recommended strains are listed below in Section 6.
A zone of inhibition occurs as a result of the diffusion of an antimicrobial agent from the specimen. All OSHA standards and rules must also be consulted and followed.
The precautions are ancillary to the testing procedures and are not intended to be all inclusive. The Parallel Streak Method has proven effective over a number of years of use in providing evidence of antibacterial activity against both Gram positive and Gram negative bacteria. Avoid contamination by using good sterile technique in plating and transferring. Your comments will receive careful consideration at a meeting of the responsible technical committee, which you may attend.
Wear safety glasses in all laboratory areas. The criterion for passing the test must be agreed upon by the interested parties. BoxManassas VA ; tel: AATCC grants you a license as follows: This is not necessary if prepared, dehydrated medium is used.
Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity. Some of the bacteria used in this test are pathogenic; i. The test Method determines antibacterial activity of diffusable antimicrobial agents on treated textile materials.
This test method is not for resale. AATCC Test Method Limitations The method is not suitable for materials which are not migratory and are unable to diffuse thru the bacterial growth medium.
Notes and References Safety Precautions reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in methkd associated environment. Foreword The Parallel Streak Method has filled a need for a relatively quick and easily executed qualitative method to determine antibacterial activity of diffusable antimicrobial agents on treated textile materials.
Culture Medium mm length permits the specimens to lie across 5 parallel inoculum streaks each of diminishing width from about 8 mm to 4 mm wide. Rectangular specimens cut 25 – 50 mm They can be any convenient size. Users of this test method are expressly advised that determination of the validity of any 1477 patent rights, and the risk of infringement of such rights, are entirely their own responsibility. Until a precision statement is generated for this test method, use standard statistical techniques in making any comparisons of test results for either within-laboratory or between-laboratory averages.
Check culture purity by making streak plates periodically and observing for a single species-characteristic type of colonies. After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen.
AATCC Antimicrobial Testing Lab – Accugen Laboratories, Inc.
Wear protective clothing and respiratory protection that prevents penetration by the bacteria. Report of results will include an observation of zones of inhibition and growth under the specimen if present. Adjust to pH 6. This may be accomplished more easily by pressing the specimen to the agar surface with a biological section lifter or with a spatula which has been sterilized by flaming and then air cooled immediately before use.
AATCC 147 – Antimicrobial Fabric Test
At the conclusion of two weeks, make a fresh transplant from stock culture. The results of using this procedure have been demonstrated by Committee RA31 to be reproducible by various laboratories working with materials containing residual amounts of antibacterial agents as determined by chemical assay after multiple standard washings.
Therefore, when the intent is to demonstrate bacteriostatic tfst by the diffusion of the antibacterial agent through agar, Method fulfills this need. Plug and sterilize at kPa 15 psi for 15 minutes. Many standard textile finishing chemicals, especially crease resistant and permanent press reagents, will often give strong antibacterial activity even after many washes.
If you feel that your comments have not received a fair hearing, you should make your views known to Christopher S.
The size of the zone of inhibition and tfst narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings.
The test is followed in the cases where the antibacterial agent is migratory i. Therefore, every necessary and 5. Take care not to break the surface of the agar while making the streaks.
The average width of a zone of inhibition along a streak on either side of the test specimen may be calculated using the following equation: Antibacterial Activity Assessment of Textile Materials: Maintenance of Culture of Test Organisms 8. Individual reprints single or multiple copies metho this method may be obtained by contacting AATCC at the above address or tel: Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7.
Uses and Limitations 1. The hard copy print may only be distributed to other employees for their internal use within your organization. In the Parallel Streak Method, the agar surface is inoculated making it easier to distinguish between the test organism and contaminant organisms which may be present on the unsterilized tezt. AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but is cumbersome and time consuming for routine quality control and screening tests.